We are a small biotech research lab studying the different populations of microorganisms in soil, trees and plants from Bay Area's forests.
We are using the latest genomic sequencing techniques to identify and classify the microbial populations in different soil samples. We want to know which microorganisms are usually associated with trees and plants and to elucidate their evolutionary history.
We use an Oxford Nanopore sequencer to identify the different species in microbial populations by means of 16S rRNA gene sequencing. We hope to fully sequence each species to help increase the current knowledge of microbial DNA population pools.
We will try to use qPCR to possibly quantify the different microbial species present in each sample. We want to also study the differential expression of microbial genes involved in different metabolic pathways that help trees and plants throughout their life stages.
We plan to grow redwood trees in our lab to study the effect of the different microorganisms in their physiology: how the different bacteria/fungi affect germination, growth and seed production.
We will try to isolate and grow as many microorganisms as possible, in order to fully characterize them and to generate a library of plant beneficial bacteria for possible uses in forest restoration projects.
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